Giemsa stains of the major morphological stages throughout the IDC are shown with the percent representation of ring, trophozoite, or schizont stage at every time point. The two-hour invasion window during the initiaion of the bioreactor culture is indicated (grey area). (A). Example expression profiles for three genes, erythrocyte binding antigen 175 (EBA 175), dihydrofolate reductase- thymidylate sythase (DHFR-TS), and adenosuccinate lyase (ASL) are shown with a Loess fit of the data (grey line) (B-D). MAL6P1.147, the largest predicted ORF in the plasmodium genome is respresented by 14 unique DNA oligonucleotide elements. The location of each of the oligonucleotide elements within the predicted ORF and the corresponding individual expression profiles are indicated (oligo 1-14). A red/green colorimetric representation of the gene expression ratios for each oligonucleotide is shown below the graph. The pair-wise Pearson correlation for these expression profiles is 0.98 +/- 0.02 (E). The percentage of the power in the maximum frequency of the Fast Fourier transform power spectrum was used as an indicator of periodicity. A histogram of these values reveals a strong bias towards single frequency expression profiles (F) indicating that the majority of P. falciparum genes are regulated in a simple periodic manner. This bias is elimnated when the percent power was recalculated using random permutations of the same dataset (inset). For reference, the locations of EBA175 (B), DHFR (C), and ASL (D) are shown.