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PAPER FIGURES:
Figure 1 Figure 1 (344 KB)
IDC Transcriptomes for three Plasmodium falciparum strains.
The phaseograms depict the 48-hour progression of gene expression throughout the IDC from the early ring stage to merozoite invasion (left to right) for the 3D7, Dd2 and HB3 strains of P. falciparum. The profiles have been ordered (top to bottom) by the phase of gene expression during the IDC and demonstrate a highly similar cascade across the three strains. Depicted above the phaseograms (in grayscale) is the relative percentage of the three morphological stages -- ring, trophozoite (troph), and schizont.

File in Portable Document Format (PDF), 2.8 MB


Figure 2 Figure 2 (228 KB)
Inter-strain differences in gene expression are rare during the IDC.
The differences in gene expression profiles as measured by Pearson correlation and the fast Fourier transform (FFT) phase are low between all strains measured here. (A) Depicts the distribution of the Pearson correlation between gene expression profiles from the 3D7 and HB3 datasets. 70% of the correlations are greater than 0.7. The inset plots the nearly linear relationship for most of the FFT phases for these two strains. The largest differences in phase are within the red circles. The arrows point to a gene with a significant change in phase between 3D7 and HB3 (MAL13P1.344) and another gene with virtually no difference (PFI1740c). The Pearson correlation and phase change between 3D7 and HB3 for these two genes are shown with the expression profiles from all three strains (B). Northern blot of MAL13P1.344 (C) shows mRNA transcript levels at the ring (R) and schizont (S) stages of development for the 3D7, Dd2 and HB3 strains of P. falciparum. Transcription of this gene in HB3 is out of phase with that measured in 3D7 and Dd2.

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Figure 3 Figure 3 (187 KB)
Multiple var genes are expressed during the IDC.
The gene expression profiles of all var genes measured for the 3D7 strain along with the respective profiles from Dd2 and HB3 are displayed. The genes are organized according to var gene group as defined by Lavstsen et al.

File in Portable Document Format (PDF), 233 KB